Gene expression dynamics during the embryonic development of mouse and chick telencephalon

In mouse, a vesicle forms at the anterior part of the developing embryo at 9.5 day/stage (M9.5), which further develops into telencephalon at M10.5 day/stage. The equivalent stages in chick embryo are referred to as HH17 and HH24. We sequenced RNA populations from telencephalic region at the early (M9.5 and HH17) and late stages (M10.5 and HH24) of mouse and chick embryo. Four samples were sequenced for each stage of telencephalon development. The resulting RNA sequencing reads were used to assemble transcripts and for counting their abundance. The read counts for each transcript then used to compute its differential expression between M9.5 and M10.5 stages in mouse. Likewise, each chick transcript was compared between H17 and HH24 stages. The raw read counts for transcripts and their differential expression results between early and late stages of development are provided here.

在鼠类胚胎中，于9.5天/发育阶段（M9.5）的前端形成囊泡，该囊泡进一步发育为端脑，至M10.5天/发育阶段。而在鸡胚中，相应的阶段被称为HH17和HH24。本研究对鼠和鸡胚端脑区域的RNA群体进行了测序，分别于早期（M9.5和HH17）及晚期（M10.5和HH24）阶段进行。针对端脑发育的每个阶段，均进行了四个样本的测序。得到的RNA测序读数被用于转录本的组装及其丰度的计数。随后，基于每个转录本的读数计数，计算其在鼠类M9.5和M10.5阶段间的差异表达。同样，每个鸡胚转录本在H17与HH24阶段间进行比较。转录本的原始读数计数及其在早期与晚期发育阶段间的差异表达结果，现已提供。