Optimization of Hydrolases Production (Dataset).pdf

The saccharification of
biomass with hydrolases from fungi is considered as an efficient and
sustainable approach to obtain fermentable sugars. The expression of these
hydrolytic enzymes by fungi is mainly by a substrate-induced mechanism, which
depends on prevailing environmental conditions. Trametes polyzona, a white rot fungus, is regarded as one of the
most efficient producers of hydrolases for the hydrolysis of biomass. This
study sought to examine and optimize cultivation conditions for the efficient
production of hydrolases by Trametes
polyzona (T. polyzona). Hydrolase production by T. polyzona was examined using
parametric optimization. Crude enzyme extracts obtained under varying
cultivation conditions were profiled for endoglucanase, betaglucosidase,
exoglucanase, xylanase and amylase activities to determine the optimal conditions
for hydrolase production. Maximum hydrolase production was achieved by
solid-state cultivation of T. polyzona
on cassava peels at a moisture content of 60%, pH 6, supplemented with 0.3%
urea and 0.03% Mg2+, and incubated at 30 oC for 14 days.
A solid to liquid ratio of 1:2 was found to be ideal for the solid-liquid
extraction of enzymes. This study therefore proves that optimization of
cultivation conditions is an efficient approach to enhance the production of
hydrolases required to breakdown lignocellulosic biomass by T. polyzona.