Untargeted Metabolomics and Quantitative Label-free Proteomics Analysis of Whole Milk Protein from Chinese Native Buffaloes

This study aimed to analyze metabolite abundances and proteome differences between Binglangjiang buffalo milk (BBM) and Dehong buffalo milk (DBM). Untargeted ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), label-free quantitative proteomics approaches, and bioinformatics analyses including Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) were performed.