Genome-wide identification of target genes for the transcription factors Nkx2.2 and Zfp488 in differentiating CG4 cells as a model for differentiating oligodendrocytes

In an attempt to validate the CG4 cell line as a model for oligodendrocytes, we determined the expression profiles in both cell types and the alterations during differentiation and found a striking overlap. Using CG4 cells as a model system for oligodendroglial cells, we generated several Nkx2.2 and Zfp488 overexpressing CG4 lines and compared their expression profile under differentiating conditions to regular CG4 cells to identify target genes of the two transcription factors in a genome-wide and unbiased approach. Several target genes were shared between Nkx2.2 and Zfp488, among them Gpr37.为验证CG4细胞系可作为少突胶质细胞的研究模型，本研究检测了两类细胞的表达谱及分化过程中的表达变化，发现二者存在显著重叠。以CG4细胞作为少突胶质细胞的模型系统，我们构建了多个过表达Nkx2.2与Zfp488的CG4细胞系，并在分化条件下将其与普通CG4细胞的表达谱进行对比，以通过全基因组无偏筛选的方式鉴定这两种转录因子的靶基因。Nkx2.2与Zfp488共享多个靶基因，其中包括Gpr37。