Gene expression profiles of ovarian endometriotic cyst stromal cells treated with tucidinostat

We previously demonstrated that the expression of histone deacetylase (HDAC) 10 is elevated in ovarian endometriotic cyst stromal cells (ECSCs) compared to normal endometrial stromal cells (NESCs). HDAC10 inhibitor, tucidinostat (10 µM) inhibited the cell proliferation of ECSCs and induced the apoptosis and G0/G1 cell cycle arrest of these cells. GThe objective of this study to unveil the role of HDAC10 in the pathogenesis of endometriosis. We cultured ECSCs with an HDAC10 inhibitor, tucidinostat (10 µM), isolated the total RNAs, and subjected to next-generation RNA sequencing to identify the target genes of HDAC10. Gene Ontology analysis identified upregulated genes. We will introduce these genes into endometriosis stromal cells and examine whether they induce cell proliferation inhibition and apoptosis promotion similar to HDAC10 inhibitors. This will help us evaluate whether HDAC10 inhibitors are promising candidates for endometriosis treatment. We found that somatostatin receptor 2 (SSTR2), dachshund homolog 11 (DACH1), and interferon regulatory factor 6 (IRF6) are the possible target genes of HDAC10. Overall design: ECSCs isolated from 4 patients were placed on 10-cm culture plates (Corning, New York, NY, USA) and incubated with or without tucidinostat (10 ?M, Cat. No. S8567, Selleckchem, Houston, TX, USA) for 48 h. Then, total RNA was extracted with an RNeasy Mini kit (Qiagen, Valencia, CA, USA). The quality of the extracted RNA was confirmed by measuring the absorbance at 230 nm, 260 nm, and 280 nm using a spectrophotometer (NanoDrop 2000, Thermo Scientific, Wilmington, DE, USA) and by an Experion System (Bio-Rad Laboratories, Hercules, CA, USA). The samples were then subjected to RNA-sequence analyses .