Differential transcription in E. coli WT vs. fum null mutants in response to DNA damage induction

The objective was to study the transcriptional profiles of fum null mutants vs the WT strain via RNA-seq, in order to find differentially expressed genes (DEGs, up or down regulated) in the WT strain in response to MMS treatment (0.35% [v/v],30 min), and more importantly in response to MMS+a-KG (0.35% [v/v], 30 min +25mM a-KG when indicated), compared to the mutant strains. Overall design: 15 samples (with three biological replicates=overall 45 samples), refrece sample is the WT (BW25113) trated and untreated