High-throughput propagation of human prostate tissue from induced-pluripotent stem cells.

We demonstrate a new high-throughput model where rapidly proliferating and easily handled induced pluripotent stem cells enable generation of human prostate tissue in vivo and, for the first time, in vitro. Using a co-culture technique with urogenital sinus mesenchyme, we recapitulated the in situ prostate histology, including the stromal compartment and the full spectrum of epithelial differentiation. Examination of transcripts in IPSC, Definitive Endoderm and Prostate Pro Organoids at 12 Weeks through RNA-seq analysis