RNAseq analysis of raf18/20/24 and srk2abgh mutant under dehydration stress

We are interested in osmotic stress signaling in plants. We revealed that RAF18/20/24 activated subclass I SnRK2s which regulate plant growth under osmotic stress conditions in Arabidopsis. So, we performed transcriptomic analyses of arabidopsis raf mutant and srk2abgh mutant following a dehydration stress treatment using RNAseq analysis to reveal a functional relationship between RAF18/20/24 and subclass I SnRK2s. We used 12d-old whole seedlings of wild type (Col-0), raf mutant or srk2abgh mutant plants grown on GM agar plates following a dehydration stress for 5 hours or untreated as materials for RNAseq analysis. We also have three biological replicates for this analysis.