RNA-seq of biotin-labelled-miR190b pulldown
收藏NIAID Data Ecosystem2026-04-30 收录
下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP364259
下载链接
链接失效反馈官方服务：
资源简介：
T-47d breast cancer cells were transfected in 6 test/control replicates with biotinylated miR-190b mimic or biotinylated mimic negative control by Qiagen. After 48h cells were lysed and miRNA:mRNA complexes were pulled-down by double precipitation. Firstly by pulling down Ago2 with Dynabeads G with rad Ago2 antibody and secondly via streptavidin coated magnetic beads. The precipitation was carried out according to manufacturer protocol. RNA from the eluted samples was then isolated using phenol-chloroform-isoamyl alcohol mixure and suspended in RNAse free water. This experiment was implemented to assess direct targets bound by miR-190b in the ER-positive breast cancer subtype T-47d. Overall design: Examination of direct miR-190b targets in ER-positive breast cancer
创建时间：
2022-03-27
