SIRT1 impact on global gene expression in the brain

We compared expression of genes in brains of SIRT1 brain-specific knockouts (BSKO) to those of wild-type littermate controls (WT). RNA was isolated from whole brain tissues of SIRT1 brain specific knockout mice (BSKO) and their wild-type littermates (WT). 3 months old males were used, four biological replicates per genotype were used. RNA was verified for integrity and processed according to Affymetrix guidelines, using kits and reagents supplied by Affymetrix. Total of 8 Affymetrix.GeneChip.Mouse430A_2 chips single channel were used.本研究针对SIRT1脑特异性敲除（brain-specific knockout, BSKO）小鼠与野生型同窝对照（wild-type littermate control, WT）小鼠的大脑组织，开展了基因表达谱对比分析。实验取材于两类小鼠的全脑组织，从中分离总RNA。本次实验选用3月龄雄性小鼠，每种基因型设置4个生物学重复样本。所提取的RNA经完整性验证后，严格遵循Affymetrix公司的实验指南进行后续处理，所用试剂盒及试剂均由Affymetrix提供。本次实验共使用8张Affymetrix GeneChip Mouse430A_2单通道基因芯片。