Proteomics analysis of bile acids modulation on rat colonic expression of water, electrolyte and drug transporters

Bile acid diarrhoea is a chronic condition with increased delivery of bile acids to the colon causing diarrhoea. In this study we used a rodent model of bile acid malabsorption to investigate the general impact on increased levels of bile acids on mRNA- and protein-expression in colon epithelial cells. Twelve male Wistar Munich rats were housed in metabolic cages and fed control or bile acid-mixed (1% w/w) diets for ten days. After euthanasia, colonic epithelial cells were isolated using Ca2+ chelation and processed for mass spectrometry-based proteomics to quantify changes in protein expressions.