Transcriptomic analysis of muscular layer of the uterus in mares with endometrosis

Endometrosis significantly decreases the reproductive potential of mares, and thus, mechanisms of its etiology and pathogenesis are still under intense investigation. We hypothesized that changes occurring in the endometrium of mares with endometrosis are accompanied by changes in the myometrium. For this reason, we aimed to determine and compare transcriptomic profiles of the myometrium of mares in the progression of endometrosis (RNAseq, bioinformatics analysis, qPCR). The myometrium was collected from mares during the mid-luteal phase of the estrous cycle with endometrium category I (n=5), category IIA (n=5), category IIB (n=4), and category III (n=5), based on the Kenney and Doig classification system.