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Trypanosoma brucei strain:Lister 427 Genome sequencing

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下载链接:
https://www.ncbi.nlm.nih.gov/sra/SRP188221
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资源简介:
To provide evidence that the Trypanosoma brucei vault RNA is transcribed by RNA polymerase III (Pol III), we performed ChIP-seq. One of the alleles for the largest subunit of Pol III (Tb927.10.2780) was tagged with a Ty1 epitope (EVHTNQDPLD)-mCherry fusion and the second allele was deleted. After formaldehyde cross-linking and immunoprecipitation of chromatin fragments with monoclonal antibody against the Ty1 epitope, libraries for high-throughput sequencing were generated and processed on the Illumina platform.
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2020-03-08
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