CIL:41356, Mus musculus, adenocarcinoma. In Cell Image Library
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Mouse adenocarcinoma cells were grown on gold grids coated with cellulose nitrate, incubated with 270 nm diameter gold-coated silica beads, plunge frozen in liquid ethane, transferred to a Gatan 630 cryoholder, and imaged at -170C in the frozen hydrated state using the X-ray microscope at the Helmholz Zentrum, Berlin, Germany. The microscope uses partially coherent illumination and a zone plate objective, and allows the specimen to be observed over a range of tilts. The images were aligned using the gold beads as fiduciary markers, and reconstructed using a reciprocal space algorithm.
See: G Schneider et al. 2010 Three-dimensional cellular ultrastructure resolved by X-ray microscopy. Nat Meth 7:985-987.
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UC San Diego Library Digital Collections
创建时间：
2021-04-15
