Cell fusing agent virus sequencing

Aedes aegypti mosquitoes are vectors for such medically important arboviruses as dengue or Zika viruses. However mosquitoes are also naturally infected with other viruses that belong to genus Flavivirus, that can only replicate in insect cells - insect specific flaviviruses. One of the insect specific flaviviruses, cell fusing agent virus (CFAV) had been isolated from the 4th generation of Thai Ae. aegypti colony. The goal of this project was to sequence full genome of CFAV isolated on C6/36 cell line from mosquito homogenates. To omit potential coisolation of other viruses from mosquito homogenates, we prepared an additional CFAV stock directly from CFAV genomic RNA. In addition, we sequenced cDNA from naive C6/36 supernatant to verify its virus content.