RNA-Seq analysis of skeletal muscles of mice with chronic or acute infections of LCMV Armstrong and LCMV clone 13

Chronic but not acute infections cause cachexia, characterized by bodyweight reduction and muscle loss. The underlying mechanisms remain incompletely understood. It is also not clear if muscle, in turn, regulates antiviral immune responses. In this study, we use two infection mouse models, one is acute and the other is chronic (described in PMID6332167 and PMID17950003), to study the global transcript profiles in muscles during infections. To understand the global gene expression profiles in muscle during acute and chronic infections, we harvest quadriceps muscle from C57BL/6 mice infected with LCMV Armstrong and LCMV clone 13, followed by RNA extraction and RNA sequencing (RNA-seq) analysis.