EGAS00001002104-sc-2020-08-05T15:29:39Z - samples

This dataset was used to characterise T cell gene
expression and clonality at sites of active inflammation within
the joints of psoriatic arthritis (PsA) patients, and to compare
these results with T cells from the peripheral blood of those
same patients. Freshly sorted CD45RA negative CD3+CD4+ and
CD3+CD8+ single cells from four patients were individually flow
sorted into 96-well full-skirted plates (Eppendorf) containing
10µL of a 2% Dithiothreitol (DTT, 2M Sigma-Aldrich), RTL lysis
buffer (Qiagen) solution. Cell lysates were sealed, mixed and
spun down before storing at -80 ºC. Paired-end multiplexed
sequencing libraries were prepared following the Smart-seq 2
protocol using the Nextera XT DNA library prep kit (Illumina). A
pool of barcoded libraries from four different plates were
sequenced across two lanes on the Illumina HiSeq 2500.EGA dataset EGAD00001006342