Pediatric Medulloblastoma RNA and microRNA sequencing

Medulloblastoma the most common malignant brain tumor in children is divided into four tumor subgroups. The miRNA-mRNA regulatory network in Medulloblastoma is far from being fully understood. Our aims were to investigate miRNA expression regulation in Medulloblastoma subtypes, to assess miRNA-target relationships, and to identify miRNA biomarkers for rapid, specific and cost effective subgroup diagnosis. With these aims, integrated mRNA and miRNA expression analysis was performed on primary tumor samples collected from 9 Medulloblastoma patients (1 - WNT sub-group, 1 - SHH sub-group, 2 - sub-group 3 and 5 - sub-group 4) using small RNA sequencing and RNA-seq.