Select chromatin complexes are essential drivers of pathogenic enhancer activity and gene expression in Ewing sarcoma [RNA-seq]

Using RNA-seq we assessed the EWS:FLI1 MEDIATED gene expression changes occuring following perturbation of EWS::FLI1, MLL3/4 and p300/CBP. Overall design: Using previously described EWS::FLI1 degrader cell lines (A673-dHALO and EWS::502 dTAG) we assessed the EWS::FLI1 driven gene expression changes compared to MLL3/4 genetic deletion and p300/CBP perturbation using small molecule inhibitor A485 and degrader dCBP1.