Long non-coding RNAs expression study of a Rett syndrome mouse model

In order to identify some lncRNA potentially involved in Rett syndrome we performed a mouse lncRNA expression microarray on whole brain samples coming from wild-type and MeCP2 Knock-out littermates. We found 2 lncRNAs directly bound by MeCP2 and upregulated in KO samples. We then focused on AK081227 because it overlaps with Gabrr2. The expression of this GABA receptor and the overlapping AK081227 are inversely correlated in thalamus, suggesting the long non-coding is regulating his own host. Whole brain total RNA was extracted from two Wild type mice (P60) and two MeCP2 KO littermates (P60)为筛选潜在参与雷特综合征（Rett syndrome）的长链非编码RNA（long non-coding RNA，lncRNA），我们针对来自野生型（wild-type）与MeCP2基因敲除（MeCP2 Knock-out）同窝仔鼠的全脑样本，开展了小鼠lncRNA表达芯片检测。我们发现2个可被MeCP2直接结合且在敲除（KO）样本中表达上调的lncRNA。随后我们将研究重点聚焦于AK081227，因其与Gabrr2基因存在序列重叠区域。该γ-氨基丁酸受体（GABA receptor）与重叠的AK081227在丘脑（thalamus）中的表达呈负相关，提示这一长链非编码RNA可调控其宿主基因的表达。我们从2只出生后第60天（P60）的野生型小鼠与2只同窝出生的MeCP2基因敲除小鼠（P60）中提取了全脑总RNA。