Histone H3K4 trimethylation in rat peripheral nerve

ChIP-seq of H3K4me3 in rat peripheral nerve was used to identify transcription start sites associated with Schwann cell-expressed genes. The analysis was performed in injured and control nerve to identify injury-responsive changes in Schwann cells.本研究采用大鼠外周神经中组蛋白H3赖氨酸4三甲基化（H3K4me3）的染色质免疫共沉淀测序（Chromatin Immunoprecipitation sequencing，ChIP-seq）技术，以鉴定与雪旺细胞（Schwann cell）表达基因相关的转录起始位点。随后，分别以损伤神经与对照神经为样本进行分析，以明确雪旺细胞内的损伤应答性变化。