Next-generation sequencing of CUT&RUN of MKL-1 Merkel cell carcinoma cells treated with EPZ011989

The overarching goal of this study was to characterize mechanisms of sensitivity to EZH2 inhibitors in Merkel cell carcinoma cell lines. To that end, CUT&RUN for H3K27me3 and H3K4me3 histone marks was performed in MKL-1 cells treated with the EZH2 inhibitor EPZ011989 for 6 days. MKL-1 cells were treated in duplicate with 3 µM EPZ011989 or vehicle (1% ethanol) for 6 days with refreshment on day 3. Each sample was split into thirds and CUT&RUN was performed with antibodies against H3K27me3 or H3K4me3 or control IgG. 12 samples are analyzed.