Morphometric measurements of cranial cuticular structures and exocrine glands in Nasonia and Trichomalopsis species.

The table contains measurement data for morphometric analysis of cranial skeletal and glandular elements for Hoag et al: The cranial gland system of Nasonia spp.: a link between chemical ecology, evo-devo and descriptive taxonomy (Hymenoptera: Chalcidoidea).Figures for the description below can be accessed from the paper.Cranial morphometrics (gena height, GH, the longest perpendicular anatomical line between gena width (GW) and ventrolateral genal margin; gena width, distance between the median point on the distal margin of the clypeus and the lowest point of the compound eye, head width, HW, longest distance between the lateral eye margins and height of basal mandibular carina, BCH; Figs. 3A, B, Supplementary Fig. 1A, B, Werren et al. 2016) were measured with Fiji using anterior view screenshots of the 3D volume rendered models from 3D Slicer using the slicermorph extension. The volumes of the subcuticular space (scs: Supplementary Fig. 1C, D) were measured using 3D slicer image segmentations and Quantification/Segment Statistics (Labelmaps statistic) modules. Overall gland volume was impossible to measure as, in most cases, we were not able to differentiate gland cells from the adjacent fat body tissues (Fig. 7D–H). Volumes were calculated in Microsoft Excel by summing the surface area of each gland outline and then multiplying this value by the slice spacing of 1.22 μm. SCV, FE, BCH were standardized with HW as a body-size proxy (Ohl and Thiele 2007) and analyzed using a Kruskal-Wallis non-parametric ANOVA and Spearman’s rank correlation.