Integrated analysis of miRNA landscape and cellular networking pathways in stage-specific prostate cancer

<strong>Figure S1.</strong> Heat map of miRNA-microarray. Expression of miRNAs differentially expressed and assessed in microarray analysis of RNA isolated from four different cell lines of prostate cancer (LNCaP, PC3, DU145, 22Rv1) compared with control cell line of prostate cancer (PrEc). The red color depicts high and green color showed a lower level of expression at p value &lt;0.01.

<strong>Figure S2A.</strong> Drug resistance by drug efflux. The internetworking relationship with miRNAs and plasma membrane protein P-glycoprotein (Pgp-plasma membrane protein). The miRNA-130a and miR-181a are downregulated in this pathway (green color) and linked with Pg and BCRP (breast cancer resistant protein). miR-133a and miR379 are involved in regulating the expression of MRP2. While miR-298, miR27a, miR331-5p and miR-130a are involved in regulating the expression of poly-glycoprotein (P-gp).

<strong>Figure S2B.</strong> Epithelial mesenchymal transition pathway. The miR-200b was downregulated during early-stage prostate cancer and was involved in inhibiting Jagged-2 (JAG2), one of the NOTCH ligands.

<strong>Figure S2C.</strong> Adipogenesis pathway. The expression of miR-326 was upregulated during the metastatic stage of prostate cancer, (red color) and involved in regulating the expression of CCAT/enhancer binding protein β (C/EBPβ), directly linked with the nuclear hormone receptor peroxisome proliferator-activated receptor-gamma (PPAR-γ).

<strong>Figure S2D.</strong> Bone metamorphosis signaling pathway. The expression of miR-140, miR-145, and miR-155 was upregulated, along with miR-140 and miR-145 were associated with modulation of gene SOX9, and miR-155 was involved in regulating the gene FOXO3A.

<strong>Figure S2E.</strong> Th1 pathway. In this pathway, the expression of miR-146a showed a higher level of expression (red color) and modulated the expression of NF-κB signaling.

<strong>Figure S2F.</strong> Th1 and Th2 pathway. In this pathway, the expression of miR-146a showed a lower level of expression (green color) and may modulate the expression of NF-κB signaling.

<strong>Table S1.</strong> List of differentially expressed miRNAs derived from LNCaP cells lines statistically significant as P &lt; 0.001.

<strong>Table S2.</strong> List of differentially expressed miRNAs derived from PC3 cells lines statistically significant as P &lt; 0.001.

<strong>Table S3.</strong> List of differentially expressed miRNAs derived from DU145 cells lines statistically significant as P &lt; 0.001.

<strong>Table S4.</strong> List of differentially expressed miRNAs derived from 22Rv1 cells lines statistically significant as P &lt; 0.001.