Assessing kidney injury induced by mercuric chloride in guinea pigs with in vivo and in vitro experiments [in vivo]

In this study we tested the ability to predict organ injury from in vivo transcriptomics data in male Hartley guinea pigs at early time points after exposure to mercury chloride (9 and 33 hours). We selected mercury chloride, a compound extensively used in animal studies for its ability to cause acute kidney damage. We treated male Hartley guinea pigs with saline solution (control), 1.00 mg/kg (low dose), and 1.50 mg/kg (high dose) to produce different degrees of injury. RNA samples for gene expression analysis were collected from the kidney at 9 and 33 hours. Number of repicates were five.