rCRUX Generated MarVer3 Marine Mammal Reference Database

rCRUX generated reference database using NCBI nt blast database downloaded in December 2022. Primer Name: MarVer3 Marine Mammal<br> Gene: 16S<br> Length of Target: 232-274<br> get_seeds_local() minimum length: 160<br> get_seeds_local() maximum length: 345<br> blast_seeds() minimum length: 124<br> blast_seeds() maximum length: 309<br> max_to_blast: 100<br> Forward Sequence (5'-3'): AGACGAGAAGACCCTRTG<br> Reverse Sequence (5'-3'): GGATTGCGCTGTTATCCC<br> Reference: Valsecchi, E., Bylemans, J., Goodman, S. J., Lombardi, R., Carr, I., Castellano, L., ... &amp; Galli, P. (2020). Novel universal primers for metabarcoding environmental DNA surveys of marine mammals and other marine vertebrates. Environmental DNA, 2(4), 460-476. https://doi.org/10.1002/edn3.72 We chose default rCRUX parameters for <em>get_blast_seeds</em>() of percent coverage of 70, percent identity of 70, evalue 3e+7, and max number of blast alignments = '100000000' and for <em>blast_seeds</em>() of coverage of 70, percent identity of 70, evalue 3e+7, rank of genus, and max number of blast alignments = '10000000'.