RNA transcriptome sequencing analysis of Control HASMCs, RSL3-induced HASMCs, and RSL3+Silibinin HASMCs

The mechanism of aortic dissection (AD) remains unclear. Several studies have demonstrated the potential protective and therapeutic applications of silibinin in cardiovascular diseases (CVDs), particularly in terms of its antioxidant, hypolipidemic, hypoglycemic, anti-hypertensive, and cardioprotective effects. The precise mechanism of how SIL impacts the occurrence and development of AD is still not completely understood. RNA transcriptome sequencing analysis was performed in control HASMCs, RSL3-induced HASMCs, and RSL3+SIL HASMCs. The HASMCs were treated with RSL3 (50 nM) for 12 h to create the ferroptosis cell model. In the RSL3+SIL HASMCs group, 10 uM SIL was used.