Sequences and thermal denaturation temperatures of LNA/DNA capture probes prepared in this study.^a

a Thermal denaturation temperatures Tm (°C)/change in Tm relative to corresponding reference duplex, ΔTm (°C). Tm values measured as the maximum of the first derivatives of the melting curves (A260 vs temperature). Reported Tm values were obtained in medium salt buffer and are averages of at least two measurements with resulting Tm ± 0.5°C. LNA nucleotides are marked with plus before the corresponding letter. Allele-specific nucleotide is underlined. 63Mer BRAF target sequences (SNP position is underlined): 5’-CATGAAGACCTCACAGTAAAAATAGGTGATTTTGGTCTAGC TACAGTGAAATCTCGATGGAGT-3’ (T1); 5’-CATGAAGACCTCACAGTAAAAATAGGTGATT TTGGTCTAGCTACAGAGAAATCTCGATGGAGT-3’ (T2). Sequences and thermal denaturation temperatures of LNA/DNA capture probes prepared in this study.a