Single cell transcriptome atlas of microenvironment in preovulation follicle. Single cell transcriptome atlas of microenvironment in preovulation follicle

The follicular microenvironment, including intra-follicular granulosa cells (GCs),are responsible for the dynamics of ovarian function. However the cellular component of follicular microenvironment as well as the different functional clusters of GCs and the other type cells in preovulatory follicle have not been extensively explored. Here we surveyed the single-cell transcriptome of follicular microenvironment around MII oocytes in six human preovulatory follicles in in-vitro fertilization. There were 6 different cell types in preovulatory follicles, including GCs, epithelial cells, and various immune cells.We identified 9 different functional clusters with different functional transcriptomic profiles in GCs and 5 different functional clusters with different functional transcriptomic profiles in macropahge.Our results provide a comprehensive understanding of the transcriptomic landscape of the preovulatory follicular microenvironment at the single-cell level. Overall design: We surveyed the single-cell transcriptome of the follicular microenvironment cells in six human preovulatory follicles in IVF. Raw data not provided due to patient privacy concerns.