Biochemical Characterization of Dimorcarpus longan Polyphenol Oxidase Provide Insights into their Catalytic Efficiency and Natural Substrate Preference. Dimorcarpus longan Polyphenol Oxidases

Longan leaves were collected in Lamphun, the northern part of Thailand, immediately transported to the laboratory and stored at -80 °C. Healthy leaves were ground under liquid nitrogen and the total RNA was isolated using the Rapid CTAB method. The PCR products on the complementary DNA of D. longan with primers enframing the ORF for prepro-DlPPO1 and pro-DlPPO1 yielded distinct bands of approximately 1800 bp and 1500 bp, respectively.