Locomotor behavior summary for the <i>pdf⁰¹</i> mutant and RNAi control lines.

Mutants lacking Pdf display several changes in locomotor rhythms as first described by Renn et al., (1999). Pdf01 mutants display higher levels of arhythmicity under DD, a shortened free-running period (p<0.0001 by t-test), and an advanced evening peak phase compared to wild-type flies (p<0.0001 by t-test). However, there was no significant difference between these genotypes in the power of the locomotor rhythm of rhythmic flies (p = 0.130) under the experimental conditions used here. All control lines displayed high levels of DD rhythmicity. The uas-Dcr2 and uas-PDFrnai elements had little if any effects on locomotor rhythms when present as single copies. However the combination of uas-Dcr2 and two copies of uas-PDFrnai resulted in an increased period and significantly delayed evening peak. One-way ANOVA of free-running period indicated a significant difference between genotypes, and a Tukey's multiple comparison test revealed a significant difference between w1118 and uas-dcr2 (p<0.05) and between uas-Dcr2/y;;uas-PDFrnai and all other genotypes. These control lines also displayed significant differences in the phase of evening activity (p<0.0001 by ANOVA), with Tukey's test revealing significant differences for all pairs of genotype except w1118 versus uas-Dcr2).