Symbiont_PCR_Climate_Data.xlsx

<b>Environmental Data Collection:</b> Microclimatic conditions at tsetse fly trapping sites were recorded using calibrated ZebraTech XH-W3001 thermohygrometers, providing temperature (±0.5°C) and humidity (±3% RH) data at 15-minute intervals, synchronized with GPS timestamps (Gao <i>et al.,</i> 2016). Sensors were mounted at 1.2m—<i>Glossina’s</i> typical flight height—and validated against NIST-traceable standards. The system captured diurnal cycles (06:00–18:00), thermal gradients (&gt;4°C between shaded/exposed areas), and RH fluctuations near water sources, critical given tsetse’s sensitivity to microclimate (Hargrove and Ackley<i>, </i>2015).