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When high uncertainty becomes the norm in entrepreneurship, entrepreneurial failure often becomes the first natural barrier that new ventures face. In dynamic environment, there is still a lack of clear answers on what strategic orientation new ventures need to adopt to improve organizational learning efficiency and achieve sustainable development. Lean startup theory believes that the entrepreneurial process is also a process where organizational cognition is constantly iterated and updated, which drives entrepreneurs to explore business opportunities through iteration learning and early customer insight. The paper aims to describe and examine the relationship between lean startup strategy, organizational iterative learning, and sustainable development of new ventures. This model is tested on the survey data of R&D departments of 325 technology new ventures in China. The research results show that: lean startup strategy can positively affect sustainable performance of new ventures; organizational iterative learning plays a mediating role in the relationship between lean startup strategy and sustainable development; market dynamics positively moderate the relationship between organizational iterative learning and sustainable development, while technology dynamics negatively moderate this relationship; furthermore, the two also moderate the process of lean startup strategy influencing sustainable development through organizational iterative learning, and that moderated mediating effect is significant. The research results reveal that entrepreneurs should deepen lean startup practices in new business layout, advocate the iteration and output of advanced knowledge, help enterprises establish proprietary knowledge barriers, and achieve sustainable development.