Microbial diversity of assimilated root secretions after treatment with autotoxin

The cultivation and production of grapevine are seriously compromised by replant disease. Root secretion is considered to play an important role in mediating interactions between plants and root microorganism. Self-toxic substance secreted by roots into the soil can not only trigger root microorganism imbalances, but also have a feedback regulation effect on root secretory properties. However, little is known how Self-toxic substance affect root secretion characteristics and the altered root secretion regulate the composition and function of root microorganisms. Here, Self-toxic substance was added to the soil of potted grapevine seedlings, and the microorganism of bacteria and fungi colonizing the root soil of grapevine which assimilated plant-derived carbon following carbon 13 carbon dioxide labeling were identified by DNA stable isotope probing and high-throughput sequencing. Then, the composition of grapevine root secretions after self-toxic substance treatment were determined by High Performance Liquid Chromatography-MS, and the mixtures of compounds that were significantly up- (Up) or down-regulated (Down) in the root secretion of self-toxic substance treated plants were added to soils to assess their effects on soil microbial community.