Gene expression signature of the three major epidermal differentiation compartments: interfollicular epidermis (IFE), hair follicle (HF) and sebaceous gland (SG)

There are multiple stem cells in adult mammalian epidermis, but the mechanisms controlling lineage specification are poorly understood. To identify gene expression signatures of the three major epidermal differentiation compartments we micro-dissected individual SG, IFE and HF from adult epidermis. The RNA was isolated from age and sex matched wild-type mice and performed transcriptome analysis with Affymetrix Exon microarrays We micro-dissected individual interfollicular epidermis (IFE), hair follicle (HF) and sebaceous gland (SG) from adult tail epidermis. The RNA was isolated from age and sex matched wild-type mice. Three biological replicates for each epidermal differentiation compartment were analyzed.成年哺乳动物表皮中存在多种干细胞，但调控其谱系特化的分子机制目前尚不清楚。为鉴定三类主要表皮分化区室的基因表达特征，我们先后从成年表皮及成年尾表皮中，分别显微分离得到单个毛囊间表皮（interfollicular epidermis, IFE）、毛囊（hair follicle, HF）及皮脂腺（sebaceous gland, SG）样本。所有样本均取自年龄、性别匹配的野生型小鼠，提取总RNA后采用Affymetrix外显子微阵列开展转录组分析。每个表皮分化区室均设置三次生物学重复并完成检测分析。