Analysis of replication profile in Geminocystis sp. NIES-3708. Geminocystis sp. NIES-3708

When bacteria with a single chromosome, such as Escherichia coli, grow rapidly in nutrient-rich media, DNA is replicated in a multifork mode, and the oriC/ter ratio becomes >2 yielding a V-shaped profile in the depth of high-throughput sequencing reads (lowest at ter and highest at oriC). For cyanobacteria possessing multiple copies of chromosomes, in which only one or a few copies are replicated from oriC, the oriC/ter ratio approximates 1.0, but still exhibits a slightly V-shaped profile in Synechococcus elongatus PCC 7942. In order to identify the replication origin in Geminocystis sp. NIES-3708, genomic DNA was extracted from the exponentially or stationary phase cultures and analyzed using an Illumina MiSeq System.