NEON Biorepository Mammal Collection (DNA Extracts)
收藏GBIF2026-05-05 更新2026-05-27 收录
下载链接：
https://www.gbif.org/dataset/66da3373-5cca-4d00-a477-af751b5ed052
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资源简介：
DNA extracts from ear punch samples collected during NEON live-trapping events used for DNA barcoding.
Sampling
3-8 mammal trapping grids per site
3 are designated pathogen grids (3 nights per bout)
Rest are designated diversity grids (1 night per bout)
Number of bouts/year dependant on site type
Core sites: ~6 bouts/year
Gradient sites: ~4 bouts/year
Sampling aligned with lunar cycle (within ±10 days of new moon)
Ear, fecal, hair and whisker samples only collected 3 bouts/year
Methods
Live capture using Sherman box traps (10 × 10 grids, 10 m spacing)
Traps opened over night and closed during the day
Individuals vouchered if found deceased or require euthanasia, otherwise processed and released
Ear punches collected once per life of an individual
Ear punch collection restricted to target species
Subset sent for DNA barcoding or pathogen analysis; remainder are archived
Preservation
Unused PCR amplified genomic extract archived (5-30 ng/uL concentration when rehydrated in 20 uL)
Stored at −80 °C in the NEON Biorepository
提供机构：
National Ecological Observatory Network
创建时间：
2020-12-05
