A single computed slice through a tomographic reconstruction of a virological synapse formed between naturally HTLV-1 infected CD4+ T cells and autologous uninfected CD4+ target cells. HTLV-1 particle
PALM was achieved using a custom wide-field microscope platform with 100x NA 1.4 objective lens or 150X TIRF NA 1.45 lens, yielding a pixel size at the CCD camera of 0.0792 or 0.0528 micrometers respe