Transcriptome analysis during the shift from dark to blue light in Arabidopsis wild type Col-0 plants.. Arabidopsis thaliana

Three-day-old wild-type Col-0 plants grown on filter paper in the dark, as described above, were exposed to blue light and harvested after 1 hour exposure. Total RNAs were extracted using Trizol reagent (Life Technologies) and purified by PureLink RNA Mini Kits (Life Technologies). Directional RNA-seq libraries were constructed using TruSeq Small RNA Sample Prep Kits and TruSeq RNA Sample Preparation Kits according to the Directional mRNA-Seq Library Prep. Manual (Illumina) and sequenced using a HiSeq sequencer (Illumina).