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Rheumatoid arthritis (RA) is an autoimmune disease characterized by joint inflammation and swelling. Several studies have demonstrated that RA fibroblast-like synovial cells (RA-FLS) play an important

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Analysis of non-specific amplification in stem-loop primer RT-qPCR

The stem-loop RT-qPCR commonly used for miRNA detection is prone to non-specific amplification. To clarify the source of non-specific amplification and characterize its sequence features, we sequenced

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The primers for stem-loop RT-qPCR used in this study.