A Genome-Scale TF-DNA Interaction Network for Transcriptional Regulation of Arabidopsis Primary and Specialized Metabolism

We measured the transcriptional changes of dark grown seedlings in which CHA19, ENAP1, LBD16 and WRI3 translocation into the nucleus are controlled by a dexamethasone-inducible glucocorticoid receptor to investigate targets of these TFs in the TCA cycle and other sectors of primary metabolism. 35S:CHA19, 35S:GR-ENAP1, 35S:GR-LBD16 and 35S:GR-WRI3 were grown in half-strength MS media for 5 days in the dark and then transferred to media with mock or 10 uM dexamethasone to be treated for 24 hr. mRNA was extracted from whole seedlings for RNA-seq libraries.