Gas lean obob GRf/fGRmKO

Muscle glucocorticoid receptor (GR) signaling regulates gene expressions in skeletal muscle. To reveal the roles of muscle GR in systemic metabolism in obesity, ob/ob background muscle-specific GR knockout (ob/ob GRmKO) mice were generated. GR Flox+/+ Cre-/- Lep+/+ (lean GRf/f), GR Flox+/+ Cre+/- Lep+/+ (lean GRmKO), GR Flox+/+ Cre-/- Lepob/ob (ob/ob GRf/f), and GR Flox+/+ Cre+/- Lepob/ob (ob/ob GRmKO) were compared. Gastrocnemius muscle from 7-week-old male mice was excised and analyzed. We used pooled RNA samples (n = 3).肌肉糖皮质激素受体（glucocorticoid receptor, GR）信号通路调控骨骼肌的基因表达。为阐明肥胖状态下肌肉GR在全身代谢中的作用，本研究构建了ob/ob背景的肌肉特异性GR敲除（muscle-specific GR knockout, GRmKO）小鼠模型。实验对以下四组小鼠进行比较：GR Flox+/+ Cre-/- Lep+/+（瘦型GRf/f）、GR Flox+/+ Cre+/- Lep+/+（瘦型GRmKO）、GR Flox+/+ Cre-/- Lepob/ob（ob/ob型GRf/f）以及GR Flox+/+ Cre+/- Lepob/ob（ob/ob型GRmKO）。采集7周龄雄性小鼠的腓肠肌（gastrocnemius muscle）组织并进行分析，本研究采用混合RNA样本（n=3）。