Next Generation Sequencing Facilitates Quantitative Analysis of Zinc treated Wild-Type or CmtR-deleted strains of Mycobacterium bovis BCG Transcriptomes

The goal of this study aims to quantitatively compare the gene expression difference in wild-type or cmtR-deleted strains of Mycobacterium bovis BCG with and without excess zinc treatment at the transcriptome level and find the underlying mechanism how could excess zinc inhibit mycobacterial growth and its relation with transcription factor CmtR. Four samples in triplicate are analyzed. The control sample is the wild-type or cmtR-deleted strains of Mycobacterium bovis BCG without zinc treatment.