Comparative analysis of various thermostability factors among 16 thermophilic-mesophilic pairs of protein.

* The PDB IDs of Thermophilic (TS), Mesophilic (MS) pair, starting from Column 2, belong to family Citrate Synthase, Malate dehydrogenase, Rubredoxin, Cyclodextrin, Glutamate dehydrogenase, L-Lactate dehydrogenase, Thermolysin, 3-Phosphoglycerate kinase, Chey protein, Methionine aminopeptidase, Endo–1,4-Beta-Xylanase, Adenylate kinase, Ferredoxin, Pyrophosphate phosphohydrolase, Manganese superoxide dismutase and Phosphofructokinase. The parameters listed in Column 1 correspond to aromatic (Aro: residues FWY), uncharged polar (UP: residues NQST), proline (Pro), hydrophobic or aliphatic (ALI: residues VILM), charged (CHG: residues DERKH) residue contents, Arg to Lys ratio (R/K), total percentage of ion-pairs (IP), aromatic-aromatic (AAI), aromatic-sulphur (ASI), cation-pi (CPI), hydrogen bonding (HB), hydrophobic (HP) interactions, proline residue percentages occurring at 2nd position of beta turns (Bt2P) and Ncap helix positions (NCap), percentage of dipole stabilized helices (Hdip), thermolabile bonds (TL) and conformationally strained residues (CS), ratio of nonpolar to polar accessible surface areas (NP/P) and percentage of loop region (Loop).**The value shown in # represents the case in which both MS and TS proteins show absence of the corresponding parameters while the values shown in ~ represents the case in which only the MS protein shows absence of the corresponding features.*** Total number of positive values calculated for each parameter; indicates number of families in which thermophilic proteins have higher preference for the parameter than their mesophilic counterparts. Numbers of ~ values are also considered while counting total number of positive values. Detailed results can be found at http://irdp.ncl.res.in/cgi-bin/result_fetch.php?ID=iCAPScase.Comparative analysis of various thermostability factors among 16 thermophilic-mesophilic pairs of protein.