Recapitulative data of microarray and QRT-PCR experiments.

Data obtained in eIF2B-mutated and OL fibroblasts for the 10 selected genes and correlation analysis between microarray and QRT-PCR data. Expression rate of microarray were calculated using the formula Mean ((Corrected G log value)i/(Corrected G log value)j) were (Corrected G log value) were calculated as explained in material and methods. i correspond to a patient’s fibroblasts and j to a control’s fibroblasts. The (Corrected G log value) between the ethanol and thapsigargin conditions were not different for the 10 selected genes which allowed us to mean them. Expression rate of QRT-PCR were calculated using the formula Mean (2∧(delta (patient)- delta (control))) where delta (patient) correspond to (Ct gene x − Ct B2M) for one patient and delta (control) to (Ct gene x − Ct B2M) for one control. The 2∧(delta (patient)- delta (control)) between the ethanol and thapsigargin conditions were not different for the 10 selected genes which allowed us to mean them. The two correlation coefficients are transformed with the Fisher Z-transform Zf  = 1/2 * ln((1+R)/(1-R) ) and the difference z  =  (Zf1 - Zf2)/SQRT(1/(N1−3)+1/(N2−3) ) is approximately Standard Normal distributed. Then the p-value of the difference has been calculated. For six genes, the determination coefficient (R2) is significantly higher in 8/10 eIF2B-mutated patient/control fibroblasts couples than in the 10 OL patient/control fibroblasts couples. The difference between eIF2B-mutated/control and OL patient/control couples is high but not significant for the HCCS and HNRNPC genes and no significant differences is observed for the KIF5B and MRPS26 genes. FDR: False Discovery Rate value.