In vivo analysis of circRNA expression alterations in response to influenza virus infection

Our study's objective was to determine circRNA expression alterations following in vivo influenza virus infection. We challenged C57BL/6 for 48 hours with IAV-PR8. Lung samples were obtained, RNA-Seq was utilized, and the circRNAs transcriptome data was compared to the control. Overall design: Ten SPF C57BL/6 mice were divided into two groups (mock-infected and IAV PR8 infected). The infected group was inoculated with 1x104 PFU/ml of IAV PR8 viral stock through intranasal instillation. Mice were euthanized, and lung samples were collected 48 hours post-infection (hpi). Mice lungs were sent for circRNAs transcriptome analysis RNA-Seq by Shanghai OE Biotech Co., Ltd. (Shanghai, China) using an Illumina NovaSeq 6000 instrument.