16S rRNA gene amplicon sequencing of microcosms originating from Stordalen Mire, Abisko (Sweden), 2022.

Global methane (CH4) emissions from thawing permafrost peatlands are expected to increase substantially in the future. Net emission of CH4 depends on the presence of more favourable terminal electron acceptors for microbial respiration, such as ferric iron (Fe(III)). In soils with high OC content, Fe(III) is often coprecipitated with organic carbon (OC). We incubated synthesized Fe(III)-OC coprecipitates together with soil from a thawing permafrost peatland (Stordalen Mire, Abisko, Sweden) within a microcosm experiment. Microbial 16S rRNA genes were amplified using primers 515F and 806R targeting the V4 region. Library preparation steps (Nextera, Illumina) and sequencing were performed using Illumina MiSeq sequencing system (Illumina, USA) at the Institute for Medical Microbiology and Hygiene (MGM) of the University of Tuebingen (Germany).