Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [ampliseq 1]. Linking CRISPR/Cas9 double-strand break profiles to gene editing precision with BreakTag [ampliseq 1]

Here we developed BreakTag, a versatile, highly parallel and scissile-aware methodology for the profiling of Cas9-induced DNA double strand break (DSBs), to identify molecular determinants influencing Cas9 incisions Overall design: Validation of bona fide off-targets nominated with BreakTag and or TTISS-seq via amplicon sequencing