Analysis of differential gene expression in the degradation of tetracycline by Sphingobacterium sp. WM1

We isolated an efficient tetracycline degrading strain Sphingobacterium sp. WM1. To investigate gene expression patterns during tetracycline degradation by strain WM1, we conducted a comparative transcriptomic analysis using cultures of strain WM1 with and without tetracycline addition. The RNA-Seq data revealed that 90.44-96.56% of the reads mapped to the genome of Sphingobacterium sp. WM1 across all samples. Differentially expressed genes (DEGs) analysis (|log2FC| >2; p < 0.01) showed that 693 genes were significantly up-regulated and 592 genes were significantly down-regulated. Control groups (WM1_CK): cultures of strain WM1 without tetracycline addition. Experimental groups (WM1_TC):cultures of strain WM1 with 50 mg/L tetracycline.