Preparations of tau exon-specific antigens and antibodies.

(A) SDS-PAGE of the purified recombinant proteins GST-E2, GST-E3 and GST-E10. The proteins were separated on a 12% SDS-PAGE and stained with 0.25% Coomassie brilliant blue. Various recombinant proteins are indicated on the top. The relative molecular weights are shown on the left. (B) Identification of the recombinant proteins GST-E2, GST-E3 and GST-E10 by Western blots with GST specific antibody. Various recombinant proteins are indicated on the top. (C) Specificity assays of the purified human tau exon-specific antibodies (Anti-tE2, Anti-tE3, and Anti-tE10) by Western blots. 2: GST-E2; 3: GST-E3; 10: GST-E10; G: GST. A commercial GST antibody is used as the control. Various antibodies were indicated on the bottom. (D) Sensitivity assays of the purified human tau exon-specific antibodies (Anti-tE2, Anti-tE3, and Anti-tE10) by an ELISA coated with the purified tau exon proteins. Left panel: Anti-tE2; Middle panel: Anti-tE3; Right panel: Anti-tE10. X-axis shows the dilution of the antiserum and Y-axis shows the P/N values. The P/N value = 2.1 is illustrated with a dotted-line.